徐州威格顿生物科技有限公司
威格顿生物
Weigedun Biotechnology
DHFR Mouse mAb
规格信息
| 规格 | 货号 |
|---|---|
| 50ul | WGD-Z-2611102 |
| 100ul | WGD-Z-2611102 |
详细参数
| 别名 | Dihydrofolate reductase |
| 基因名 | DHFR |
| UniProt ID | P00374 |
| 反应种属 | Human |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 3O8-J5-N7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 21 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Dihydrofolate reductase (DHFR) is a central enzyme in the folate metabolic pathway, essential for the de novo synthesis of purines, thymidylate, and specific amino acids. It catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, which serves as a vital one-carbon donor for the production of nucleic acid precursors required for DNA replication and cell proliferation. Structurally, DHFR contains a highly conserved active site that accommodates its substrate and cofactor, with flexible loops facilitating the hydride transfer process. Due to its indispensable role in cell division, DHFR is a primary target for antifolate chemotherapeutic agents, such as methotrexate, which competitively inhibit the enzyme to arrest the growth of rapidly dividing cancer cells. It is also the target of various antimicrobial agents, including trimethoprim. |