徐州威格顿生物科技有限公司
威格顿生物
Weigedun Biotechnology
CRISP2 Mouse mAb
规格信息
| 规格 | 货号 |
|---|---|
| 50ul | WGD-Z-2611243 |
| 100ul | WGD-Z-2611243 |
详细参数
| 别名 | Cysteine-rich secretory protein 2; CRISP-2; Cancer/testis antigen 36; CT36; Testis-specific protein TPX-1 |
| 基因名 | CRISP2 |
| UniProt ID | P16562 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 7U8-G3-U2 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 27 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Cysteine rich secretory protein 2 (CRISP2) is a member of the CAP superfamily proteins predominantly expressed in the male reproductive tract during spermatogenesis, where it becomes incorporated into developing sperm head and tail. CRISP2 plays crucial roles in multiple aspects of sperm function, including regulating calcium fluxes during sperm capacitation by modulating ion channel activity, facilitating the acrosome reaction, and establishing normal flagellum waveform for proper sperm motility. The protein contains an N-terminal domain with cell-to-cell adhesion properties and a C-terminal domain that regulates calcium RyR channels, contributing to sperm hyperactivation. CRISP2 also participates in gamete fusion through interaction with egg plasma membrane complementary sites. Deficiency in CRISP2 results in subfertility in males due to impaired sperm motility, characterized by a stiff midpiece and inability to achieve rapid progressive movement. CRISP2 has been clinically associated with human fertility, as it binds to the CATSPER1 subunit of the Catsper ion channel necessary for normal sperm function. |