徐州威格顿生物科技有限公司
威格顿生物
Weigedun Biotechnology
NELFCD Mouse mAb
规格信息
| 规格 | 货号 |
|---|---|
| 50ul | WGD-Z-2611791 |
| 100ul | WGD-Z-2611791 |
详细参数
| 别名 | Negative elongation factor C/D; NELF-C/D; TH1-like protein |
| 基因名 | NELFCD |
| UniProt ID | Q8IXH7 |
| 反应种属 | Human |
| 应用 | WB,IP,CHIP |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 2R3-K5-X4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 66 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IP-1:100; CHIP-1:100 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Negative elongation factor complex member C/D (NELFCD) is an essential subunit of the four-protein NELF complex, which also includes NELF-A, NELF-B, and NELF-E. This complex cooperates with the DRB-sensitivity inducing factor (DSIF) to induce promoter-proximal pausing of RNA polymerase II, thereby acting as a negative regulator of transcriptional elongation. NELFCD exists in at least two isoforms generated by alternative translation initiation, both of which integrate into the NELF complex within the nucleus. The transcriptional pausing mediated by NELFCD can be relieved by P-TEFb-dependent phosphorylation, allowing for highly regulated gene expression. Additionally, NELFCD is involved in HIV-1 transcription, where the NELF complex contributes to viral latency by maintaining Pol II in a paused state. Its role in fine-tuning the timing and efficiency of transcript production is fundamental to cellular gene regulation and response to external stimuli. |