Overview
| 英文名称 | EasyStep Mouse ADP(Adiponectin) ELISA Kit |
| 别名 | GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1 |
| 种属 | Mouse |
| 研究领域 | Metabolic pathway;Infection immunity;Cardiovascular biology; |
| 检测类型 | Sandwich |
| 灵敏度 | 4.75 ng/mL |
| 标准品 | 500 ng/mL |
| 检测范围 | 7.82-500 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP, and the Mouse ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse ADP. After TMB substrate solution is added, only those wells that contain Mouse ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
