Overview
| 英文名称 | EasyStep Human PSMA(Prostate specific membrane antigen) ELISA Kit |
| 别名 | Cell growth inhibiting protein 27, Cell growth-inhibiting gene 27 protein, FGCP, Folate hydrolase (prostate-specific membrane antigen) 1, Folate hydrolase 1 |
| UniProt ID | Q04609 |
| 种属 | Human |
| 研究领域 | Signal Transduction; Cancer; Metabolism |
| 检测类型 | Sandwich |
| 灵敏度 | 0.165 ng/mL |
| 标准品 | 40 ng/mL |
| 检测范围 | 0.63-40 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMA, and the Human PSMA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSMA. After TMB substrate solution is added, only those wells that contain Human PSMA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMA in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
