Overview
| 英文名称 | EasyStep Human SAA(Serum Amyloid A) ELISA Kit |
| 别名 | SA-A, SAA1, PIG4, TP53I4, Amyloid protein A, Amyloid fibril protein AA, Serum amyloid A-1 protein |
| UniProt ID | P0DJI8 |
| 种属 | Human |
| 研究领域 | Infection immunity;Kidney biomarker;Hepatology;Urology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.15 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.78-50 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
