Overview
| 英文名称 | EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit |
| 别名 | PDF, MIC1, NAG-1, PLAB, PTGFB, Macrophage inhibitory cytokine 1, NSAID-activated gene 1 protein, Placental bone morphogenetic protein, Prostate differentiation factor |
| UniProt ID | Q99988 |
| 种属 | Human |
| 研究领域 | Cytokine;Apoptosis;Tumor immunity;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 5.22 pg/mL |
| 标准品 | 3000 pg/mL |
| 检测范围 | 46.88-3000 pg/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
