Overview
| 英文名称 | EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit |
| 别名 | 3,3',5-Triiodo-L-Thyronine, Liothyronine, Cytomel, Tertroxin |
| 种属 | Rat |
| 研究领域 | Endocrinology;Autoimmunity; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.31 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
