Overview
| 英文名称 | Mouse IL-27(Interleukin 27) Microsample ELISA Kit |
| 别名 | IL-27, IL-27A, IL27p28, IL30, MGC71873, p28, |
| UniProt ID | Q8K3I6 |
| 种属 | Mouse |
| 研究领域 | Immunology;Cancer |
| 检测类型 | Sandwich |
| 灵敏度 | 19.12 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
