Overview
| 英文名称 | Simian OC/BGP(Osteocalcin) ELISA Kit |
| 别名 | BGLAP, BGP, OC, OCN, bone gamma-carboxyglutamate protein, Osteocalcin |
| UniProt ID | P02819 |
| 种属 | Simian |
| 研究领域 | Stem Cells;Signal Transduction; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.46 ng/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.78-50 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OC/BGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OC/BGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OC/BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OC/BGP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
