Overview
| 英文名称 | Rat TRACP-5b(TartrateResistant Acid Phosphatase 5b) ELISA Kit |
| 别名 | ACP5,TRAP5b,SPENCDI |
| 种属 | Rat |
| 研究领域 | Immunology;Cardiovascular |
| 检测类型 | Sandwich |
| 灵敏度 | 0.36 ng/mL |
| 标准品 | 40 ng/mL |
| 检测范围 | 0.63-40 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
