Overview
| 英文名称 | Cattle DSE(Dermatan Sulfate Epimerase) ELISA Kit |
| 别名 | DSEPI, SART2, Squamous Cell Carcinoma Antigen Recognized By T Cells 2, Chondroitin-glucuronate 5-epimerase |
| UniProt ID | P0C2H4 |
| 种属 | Cattle |
| 研究领域 | Tumor immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.112 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle DSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle DSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle DSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle DSE in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
