Overview
| 英文名称 | Mouse Tb4(Thymosin Beta 4) ELISA Kit |
| 别名 | TMSB4X, FX, PTMB4, TB4X, TMSB4, Thymosin,Beta 4,X-Linked, TB-500, Seraspenide, Hematopoietic system regulatory peptide |
| UniProt ID | P20065 |
| 种属 | Mouse |
| 研究领域 | Metabolic pathway;Endocrinology;Bone metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.91 ng/mL |
| 标准品 | 200 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Tb4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
