Overview
| 英文名称 | EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| 别名 | 25 Hydroxy Vitamin D, |
| 种属 | Rat |
| 研究领域 | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 7.7 ng/mL |
| 标准品 | 5000 ng/mL |
| 检测范围 | 78.13-5000 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
