Overview
| 英文名称 | Dog T4(Thyroxine) ELISA Kit |
| 别名 | 3,5,3',5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine |
| 种属 | Dog |
| 研究领域 | Endocrinology;Hormone metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 1.79 ng/mL |
| 标准品 | 300 ng/mL |
| 检测范围 | 4.69-300 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
