Overview
| 英文名称 | Human MaR1(Maresin 1) ELISA Kit |
| 别名 | 7(R)-Maresin 1, quinolin-7-ol, 7-hydroxychinoline, (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-7,14-Dihydroxy-4,8,10,12,16,19-docosahexaenoic acid, 7-Chinolinol, 7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid, 4,8,10,12,16,19-Docosahexaenoic acid, 7,14-dihydroxy-, (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-, 7-Quinolinol, 7-HYDROXYQUINOLINE, 7R-maresin-1, 7-hydoxyquinoline |
| 种属 | Human |
| 研究领域 | Immunology & Inflammation |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 6.2 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 2.5h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MaR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线