Overview
| 英文名称 | Rabbit NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit |
| 别名 | NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit, RELA, V-Rel Reticuloendotheliosis Viral Oncogene Homolog A |
| 种属 | Rabbit |
| 研究领域 | Apoptosis; |
| 检测类型 | Sandwich |
| 灵敏度 | 17.5 pg/mL |
| 标准品 | 3000 pg/mL |
| 检测范围 | 46.88-3000 pg/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
