Overview
| 英文名称 | Human Cot(Cotinine) ELISA Kit |
| 别名 | Cotinine |
| 种属 | Human |
| 研究领域 | Signal transduction; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.6 ng/mL |
| 标准品 | 100 ng/ml |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 2.5h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Cot. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cot. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cot in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
