Overview
| 英文名称 | Rat IP3(Inositol Triphosphate) ELISA Kit |
| 别名 | InsP3, Inositol 1,4,5-Trisphosphate, Triphosphoinositol |
| 种属 | Rat |
| 研究领域 | Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 5.73 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
