Overview
| 英文名称 | Human CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit |
| 别名 | CREB, active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, cAMP-responsive element-binding protein 1, CREB-1, cyclic AMP-responsive element-binding protein 1, MGC9284, transactivator protein |
| UniProt ID | P16220 |
| 种属 | Human |
| 研究领域 | Epigenetics and Nuclear Signaling; Immunology; Neuroscience |
| 检测类型 | Sandwich |
| 灵敏度 | 0.14 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CREB in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
