Overview
| 英文名称 | Human Acetyl-H3(Acetyl Histone H3) ELISA Kit |
| 别名 | H3 histone family member E pseudogene, H3 histone family, member A, H3/A, H31_HUMAN, H3F3, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, , HIST1H3I, HIST1H3J, HIST3H3, histone 1, H3a, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, |
| UniProt ID | P68431 |
| 种属 | Human |
| 研究领域 | Epigenetics and Nuclear Signaling |
| 检测类型 | Sandwich |
| 灵敏度 | 0.16 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Acetyl-H3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Acetyl-H3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Acetyl-H3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Acetyl-H3 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
