Overview
| 英文名称 | EasyStep Human IL5(Interleukin 5) ELISA Kit |
| 别名 | IL-5, E-CSF, ESGF, EDF, TRF, Colony-Stimulating Factor,Eosinophil, T-Cell replacing Factor, B Cell Differentiation Factor I, Eosinophil Differentiation Factor |
| 种属 | Human |
| 研究领域 | Cytokine;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.15 pg/mL |
| 标准品 | 200 pg/mL |
| 检测范围 | 3.13-200 pg/mL |
| 样本类型 | Serum, plasma, cell culture supernatants |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5, and the Human IL5 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL5. After TMB substrate solution is added, only those wells that contain Human IL5 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
