Overview
| 英文名称 | Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit |
| 别名 | ANTI-LKM1 |
| 种属 | Human |
| 研究领域 | Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 1.66 U/mL |
| 标准品 | 300 U/mL |
| 检测范围 | 4.69-300 U/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
