Overview
| 英文名称 | EasyStep Mouse VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit |
| 别名 | CD309, FLK1, VEGFR, KDR, A Type III Receptor Tyrosine Kinase, Kinase Insert Domain Receptor, Kinase Insert Domain Receptor, Fetal Liver Kinase-1 |
| 种属 | Mouse |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Tumor immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 157.5 pg/mL |
| 标准品 | 50 ng/mL |
| 检测范围 | 0.78-50 ng/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR2, and the Mouse VEGFR2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse VEGFR2. After TMB substrate solution is added, only those wells that contain Mouse VEGFR2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
