Overview
| 英文名称 | EasyStep Human PDL1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit |
| 别名 | CD274, PDL1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1, PDCD1LG1, Programmed Cell Death Protein 1 Ligand 1 |
| 种属 | Human |
| 研究领域 | Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 1.23 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDL1, and the Human PDL1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PDL1. After TMB substrate solution is added, only those wells that contain Human PDL1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
