Overview
| 英文名称 | Mouse HE4(Epididymal protein 4) Microsample ELISA Kit |
| 别名 | Epididymis Protein 4, dJ461P17.6 , EDDM4 , epididymal protein 4 , Epididymal secretory protein E4 , Epididymis specific whey acidic protein type four disulfide core , HE 4 , Major epididymis specific protein E4 , Major epididymis-specific protein E4 , MGC57529 , Putative protease inhibitor WAP5 , WAP 5 , WAP domain containing protein HE4 , WAP domain containing protein HE4 V4 , WAP four disulfide core domain 2 , WAP four disulfide core domain protein 2 , WAP four-disulfide core domain protein 2 , WAP5 , WFDC 2 , WFDC2 , WFDC2_HUMAN |
| UniProt ID | Q9DAU7 |
| 种属 | Mouse |
| 研究领域 | Ovarian cancer |
| 检测类型 | Sandwich |
| 灵敏度 | 0.071 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HE4 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
