Overview
| 英文名称 | EasyStep Human IL1R2(Interleukin 1 Receptor Type II) ELISA Kit |
| 别名 | IL-1R2；CD121b, CD121-B, IL1-R2, IL1RB, Interleukin-1 Receptor Beta, CD121 Antigen-like Family Member |
| 种属 | Human |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 12.7 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | Serum, plasma |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1R2, and the Human IL1R2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL1R2. After TMB substrate solution is added, only those wells that contain Human IL1R2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
