Overview
| 英文名称 | Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit |
| 别名 | Bsk, BSK, CG5680, D-JNK, D-junk, DBSK/JNK, dJnk, dJNK, DJNK, DJNK/bsk, Dmel\CG5680, DmJNK, dNJNK, jnk, Jnk, JNK, jnk (bsk), JNK/SAPK, Junk, p-JNK, P-JNK, pJNK, SAPKa |
| 种属 | Rat |
| 研究领域 | Enzyme & Kinase; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.148 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
