Overview
| 英文名称 | Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit |
| 别名 | p-PRKAA1, p-AMPK, p-AMPKa1, p-AMPK alpha 1 |
| 种属 | Rat |
| 研究领域 | Enzyme & Kinase |
| 检测类型 | Sandwich |
| 灵敏度 | 1.25 ng/mL |
| 标准品 | 200 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
