Overview
| 英文名称 | Mouse TNFaIP3(Tumor Necrosis Factor Alpha Induced Protein 3) ELISA Kit |
| 别名 | A20, TNF alpha-induced protein 3, Putative DNA-binding protein A20, Zinc finger protein A20 |
| UniProt ID | Q60769 |
| 种属 | Mouse |
| 研究领域 | Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.093 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFaIP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFaIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFaIP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TNFaIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
