Overview
| 英文名称 | Cattle TCPa(Alpha-Tocopherol) ELISA Kit |
| 别名 | VE, Vitamin E, α-TCP, α-Tocopherol |
| 种属 | Cattle |
| 研究领域 | Metabolic pathway;Nutrition metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.45 µg/mL |
| 标准品 | 100 µg/mL |
| 检测范围 | 1.57-100 µg/mL |
| 样本类型 | Serum, plasma and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TCPα. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TCPα. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TCPα in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
