Overview
| 英文名称 | Mouse Cys-C(Cystatin C) ELISA Kit |
| 别名 | CST3, Cystatin 3, ARMD11, Post-Gamma-Globulin, Gamma Trace, Post-Gamma-Globulin, Neuroendocrine Basic Polypeptide, Amyloid Angiopathy And Cerebral Hemorrhage |
| UniProt ID | P21460 |
| 种属 | Mouse |
| 研究领域 | Kidney biomarker;Urology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.49 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
