Overview
| 英文名称 | Human C3a(Complement Component 3a) Microsample ELISA Kit |
| 别名 | Complement Component 3a |
| UniProt ID | P01024 |
| 种属 | Human |
| 研究领域 | Infection immunity;Immune molecule;Autoimmunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 2.04 ng/mL |
| 标准品 | 500 ng/mL |
| 检测范围 | 7.81-500 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
