Overview
| 英文名称 | Chicken CRP(C Reactive Protein) ELISA Kit |
| 别名 | C-RP, PTX1, Pentraxin-Related, Pentraxin 1 |
| 种属 | Chicken |
| 研究领域 | Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.112 ng/mL |
| 标准品 | 500 ng/mL |
| 检测范围 | 7.81-500 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
