Overview
| 英文名称 | Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit |
| 别名 | CD257, TNFSF13B, BLYS, TALL1, THANK, TNFSF20, ZTNF4, B lymphocyte stimulator, Tumor Necrosis Factor Ligand Superfamily Member 13B, Dendritic cell-derived TNF-like, BAFF |
| UniProt ID | Q9WU72 |
| 种属 | Mouse |
| 研究领域 | CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.067 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
