Overview
| 英文名称 | Mouse VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit |
| 别名 | CD144, 7B4, Cadherin 5 Type 2, VE-Cadherin, Cadherin,Vascular Endothelial, CDH5, Cadherin 5 |
| UniProt ID | P55284 |
| 种属 | Mouse |
| 研究领域 | CD & Adhesion molecule;Tumor immunity;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 1.21 ng/mL |
| 标准品 | 200 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
