Overview
| 英文名称 | Rat BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit |
| 别名 | Beta 2M, BMG, B2MG, b2M, Beta-2-Microglobulin |
| UniProt ID | P07151 |
| 种属 | Rat |
| 研究领域 | Infection immunity;Kidney biomarker; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.11 µg/mL |
| 标准品 | 20 µg/mL |
| 检测范围 | 0.32-20 µg/mL |
| 样本类型 | serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
