Overview
| 英文名称 | Human sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit |
| 别名 | MAC, Membrane Attack Complex, C5b-9, Terminal Complement Complex C5b-9, C5b9 |
| 种属 | Human |
| 研究领域 | Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 12.9 ng/mL |
| 标准品 | 2000 ng/mL |
| 检测范围 | 31.25-2000 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
