Overview
| 英文名称 | Mouse MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit |
| 别名 | MIP1 Beta, CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b, CCL4L1, C C motif chemokine ligand 4 like 1, CC chemokine ligand 4L1, C-C motif chemokine 4-like, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, Monocyte adherence-induced protein 5-alpha, Small-inducible cytokine A4-like, CCL4L, SCYA4L1, CCL4L2, SCYA4L2 |
| UniProt ID | P14097 |
| 种属 | Mouse |
| 研究领域 | Cytokine;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 5.6 pg/mL |
| 标准品 | 800 pg/mL |
| 检测范围 | 12.5-800 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
