Overview
| 英文名称 | Rat AP(Aprotinin) ELISA Kit |
| 别名 | BPTI, Trasylol, Pancreatic Trypsin Inhibitor, Basic protease inhibitor |
| 种属 | Rat |
| 研究领域 | Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.47 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
