Overview
| 英文名称 | Pig CK-18(Cytokeratin 18) ELISA Kit |
| 别名 | Cell proliferation inducing gene 46 protein , Cell proliferation inducing protein 46 , Cell proliferation-inducing gene 46 protein , CK 18 , CK-18 , CK18 , CYK 18 , CYK18 , Cytokeratin 18 , Cytokeratin endo B , Cytokeratin-18 , K 18 , K18 , K1C18_HUMAN , KA18 , Keratin 18 , Keratin 18, type I , Keratin D , keratin, type I cytoskeletal 18 , Keratin-18 , Krt18 |
| 种属 | Pig |
| 研究领域 | Cancer of the liver; Viral hepatitis |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
