Overview
| 英文名称 | Simian HBb(Hemoglobin Beta) ELISA Kit |
| 别名 | HB-B, HBD, CD113t-C, Beta Globin, Spinorphin |
| 种属 | Simian |
| 研究领域 | Hematology; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 2.82 pg/mL |
| 标准品 | 500 pg/mL |
| 检测范围 | 7.82-500 pg/mL |
| 样本类型 | serum, plasma and erythrocyte lysates |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian HBb in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
