Overview
| 英文名称 | Simian Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit |
| 别名 | Xcl1 , Lptn , Ltn , Scyc1 , Lymphotactin , C motif chemokine 1 , Cytokine SCM-1 , Lymphotaxin , Small-inducible cytokine C1 |
| 种属 | Simian |
| 研究领域 | Cytokine;Tumor immunity;Infection immunity;Dermatology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.12 ng/mL |
| 标准品 | 25 ng/mL |
| 检测范围 | 0.39-25 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
