Overview
| 英文名称 | Human DDR1(Epithelial Discoidin Domain Containing Receptor 1) ELISA Kit |
| 别名 | Epithelial discoidin domain receptor 1,CD167 antigen-like family member A,Cell adhesion kinase,Discoidin receptor tyrosine kinase,HGK2,CD167a,DDR1,Mammary carcinoma kinase 10, |
| UniProt ID | Q08345 |
| 种属 | Human |
| 研究领域 | Cardiovascular;Immunology;Metabolism;Signal Transduction |
| 检测类型 | Sandwich |
| 灵敏度 | 0.061 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
