Overview
| 英文名称 | Mouse FA(Folic Acid) ELISA Kit |
| 别名 | VB9, Vitamin B9, Folacin, Folate, V, Itamin M, Vitamin M, Vitamin Bc, Pteroyl-L-Glutamic Acid, Pteroyl-L-Glutamate |
| 种属 | Mouse |
| 研究领域 | Hematology;Reproductive science;Nutrition metabolism; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 1.42 ng/mL |
| 标准品 | 500 ng/mL |
| 检测范围 | 7.81-500 ng/mL |
| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse FA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FA in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
