Overview
| 英文名称 | Human HMGB-2(High Mobility Group Protein B2) ELISA Kit |
| 别名 | High mobility group protein B2,HMGB2,HMG2,High mobility group protein 2 |
| UniProt ID | P26583 |
| 种属 | Human |
| 研究领域 | Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 26.3 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | Serum, plasma and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
