Overview
| 英文名称 | Chicken IgM(Immunoglobulin M) ELISA Kit |
| 别名 | IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region |
| UniProt ID | P01875 |
| 种属 | Chicken |
| 研究领域 | Infection immunity;Immune molecule;Hematology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.71 µg/mL |
| 标准品 | 100 µg/mL |
| 检测范围 | 1.57-100 µg/mL |
| 样本类型 | serum, plasma, and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
