Overview
| 英文名称 | Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit |
| 别名 | NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, Nitric Oxide Synthase 2, Inducible |
| UniProt ID | Q06518 |
| 种属 | Rat |
| 研究领域 | Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.62 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
