Overview
| 英文名称 | Rat PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit |
| 别名 | PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3, PPARg, Peroxisome Proliferator Activated Receptor Gamma |
| UniProt ID | O88275 |
| 种属 | Rat |
| 研究领域 | Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.059 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
