Overview
| 英文名称 | Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit |
| 别名 | IL-2RA,CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen, IL2Ra, Interleukin 2 Receptor Alpha |
| 种属 | Human |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 6.7 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
